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Quick Details

  • CAS No.: 28211-04-3
  • Other Names: e-polylysine
  • MF: (C6H12N2O)n
  • Place of Origin: China (Mainland)
  • Type: Preservatives

Packaging & Delivery

Packaging Details 0.25kg, 0.5kg HDPE bottles with integral, tamper-proof seals, or 20 kg, 25kg cartons or based on clients requirement

Specifications

Anti fungi,G+/- bacteria,yeasts and moulds for foods

ε-Polylysine can greatly inhibit the growth of bacteria, yeasts and moulds bacteria, especially to the Gram+ bacteria which can’t easily inhibit by others. As a good preservative, ε-PL is characterized as being high safety, water-soluble, stable at high temperatures, widely usage, non prejudice to the taste, antibacterial in neutral or subacidity conditions. Because of the stability at high temperatures, it can inhibit some heat-resistant bacillus after heat treatment and some tadpole-form no contractile siphovirus bacteriophage as well.

 

Antibacterial Spectrum

  

Table 1  ε-PL MIC Test (minimum inhibition concentration)

  

Different Types of Microorganisms

 

Bacteric

MIC

mg/Ltr

 

pH

 

Fungi

Aspergillus niger IFO4416

250

5.6

Trichophyton mentagrophytes IFO7522

60

5.6

 

 

 

Yeast

Candida acutus IFO1912

6

5.0

Phaffia rhodozyma IFO10129

12

5.0

Pichia anomala IFO0146 

150

5.0

Pichia membranaefaciens IFO0577

< 3

5.0

Rhodotorula lactase IFO1423

25

5.6

Sporobolomyces roseus IFO1037

< 3

5.0

Saccharomyces cerevisiae

50

5.0

Zygosaccharomyces rouxii IFO1130

150

5.6

 

 

 

Gram+ bacteria

Geobacillus stearothermophilus IFO12550

5

7.0

Bacillus coagulans IFO12583

10

7.0

Bacillus subtilis IAM1069 

< 3

7.3

Clostridium acetobutylicum IFO13948

32

7.1

Leuconostoc mesenteroides

50

6.0

Lactobacillus brevis IFO3960

10

6.0

L1 plantarum IFO12519

5

6.0

Micrococcus luteus IFO12708

16

7.0

Staphylococcus aureus IFO13276

12

7.0

Streptococcus lactis IFO12546

100

6.0

 

 

Gram- bacteria

Raoultella planticola IFO3317

8

7.0

Campylobacter jejuni

100

7.0

Escherichia coli IFO13500

50

7.0

Pseudomonas aeruginosa IFO3923

3

7.0

Salmonella typhymurium

 

 

 

  

As can be seen, ε-PL has wide range of antimicrobial spectrum with minimum inhibitory concentration of bacteria less than 100μg/ml, and to Fungi, the MIC is higher.

   

Antibacterial Mechanism

  

     ε-PL is in high aggregation state of multivalent cations, which can damage the cell membrane structure of microorganisms, causing cell material, energy and information transmission interrupted. Its combination with the ribosomes within the cell affects the synthesis of biological macromolecules, eventually leading to cell death.

  

Safety

  

It had been approved by FDA for use as an antimicrobial agent in cooked rice or sushi rice at levels up to 50 milligrams per kilogram (mg/kg) of rice. The results of an acute toxicity study and bacterial assays for mutagenicity show that ε-Polylysine was non-toxic and there was no evidence for mutagenic activity of ε-Polylysine. So ε-Polylysine is absolutely safe to be used as food preservative.  

 

Range of Application 

 

With features of solubility in water, good heat tolerance and extensive antimicrobial against fungi, ε- PL can be used under various conditions. A test for thermal stability and pH test using scope of ε-PL was carried out and the results are shown in Table 2 and Table 3

 

 

 

Table-2  Effect of temperature on the Minimum Inhibition Concentration (MIC) of ε-Polylysine against Growth of Escherichia coil

 

 

 

Treatment Condition

MIC (mg/Ltr)

Untreated

50

80 °C,60 min

50

100 °C,30 min

50

120 °C,20 min

50

 

 

 

Table 3  PH on ε-PL MIC

 

 

 

Bacteria for Test

MIC  (mg/Ltr)

 

pH =5.0

pH =6.0

pH =7.0

pH =8.0

Bacillus subtilis

3.0

3.0

3.0

3.0

Bacillus cereus

25.0

100.0

50.0

12.5

Escherichia coli

25.0

25.0

50.0

50.0

Staphylococcus aureus

12.5

25.0

12.5

< 6.3

  

 

No Influence on Food Flavor

  

 

As a food preservative, ε-PL does not affect food flavor.  When added to food, ε-PL and dextran will have Maillard reaction after high temperature disinfecting because of its excellent

 

heat-resistance property. Not only can it maintain the effectiveness of antimicrobial, but also it has strong emulsifying properties, which is unacted whether the food is under circumstances of PH7 or 1 mol/L NaCl. Therefore, ε-PL as food additive has a dual function of emulsification and fresh-keeping.

 

 

 

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